Molecular mechanism underlying differentiation of HL-60 cells induced by hexamethylene bisacetamide.
- Author:
Xia REN
1
;
Pei-E WEN
;
Wei-Hua YANG
;
Tian-Hua TANG
;
Hai-Quan REN
;
Zhi-Yong ZHANG
;
Hai-Tao ZHAO
;
Hua FAN
;
Gao-Juan QIAO
;
Guo-Heng JIANG
Author Information
1. Institute of Basic Medical Sciences, Shandong Academy of Medical Sciences, Jinan 250062, Shangdong Province, China.
- Publication Type:Journal Article
- MeSH:
Acetamides;
pharmacology;
Antineoplastic Agents;
pharmacology;
Cell Cycle;
drug effects;
Cell Differentiation;
drug effects;
genetics;
Cell Proliferation;
drug effects;
HL-60 Cells;
Humans
- From:
Journal of Experimental Hematology
2008;16(5):1030-1034
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to investigate the effect of hexamethylene bisacetamide (HMBA) on differentiation of HL-60 cells and its possible molecular mechanism. HL-60 cells were co-cultured with different concentrations of HMBA (0.5, 1, 2 mmol/L) for 4 days, then the proliferation was assayed by MTT at different time points. Wright-Giemsa staining was used to observe the change in morphology. Cell differentiation antigen CD11b expression and the altered distribution of cell cycle in HL-60 induced by HMBA were analyzed by flow cytometry. The expressions of c-myc, mad1, p21, p27, hTERT and HDAC1 mRNA were detected by RT-PCR. The results indicated that the proliferation of HL-60 cells was inhibited by HMBA in a time-and-dose-dependent manner. Upon 2 mmol/L HMBA treatment, the HL-60 cells arrested at G(0)/G(1) phase and differentiated into granular line in morphology, with the up-regulation of CD11b expression. The expression of c-myc and hTERT mRNA obviously down-regulated, the expression of p21, p27 and mad1 mRNA up-regulated, while there was no change of the expression of hTERT mRNA. It is concluded that effect of HMBA on the differentiation of HL-60 cells may partly contribute to switch from c-myc to mad1 expression, to up-regulate expressions of p21 and p27 mRNA, and down-regulate hTERT mRNA expression, while there is no relation with the expression of HDAC1 mRNA.
