Arsenic trioxide induces socs-1 gene demethylation in myeloma cell lines.
- Author:
Ming-Ming WANG
1
;
Qi ZHU
;
Zhi-Hong REN
;
Li-Fang ZOU
;
Hong-Ju DOU
;
Jun-Pei HU
Author Information
1. Department of Hematology, Shanghai Ninth People Hospital, Shanghai Institute of Hematology, Shanghai 200011, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Arsenicals;
pharmacology;
Cell Line, Tumor;
CpG Islands;
DNA Methylation;
Gene Expression Regulation, Neoplastic;
Humans;
Multiple Myeloma;
genetics;
Oxides;
pharmacology;
Suppressor of Cytokine Signaling 1 Protein;
Suppressor of Cytokine Signaling Proteins;
genetics
- From:
Journal of Experimental Hematology
2008;16(5):1064-1068
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to explore the effect of arsenic trioxide (As(2)O(3)) on the methylation status of socs-1 gene in multiple myeloma cell lines U266, RPMI8226. The cell viability was assayed by MTT method. The methylation status of socs-1 gene was detected by methylation specific PCR. The expression of socs-1 gene mRNA was determined with real-time PCR. The cell apoptosis was analyzed by flow cytometry. The results indicated that hypermethylation of CpG island of socs-1 gene was observed without expression of socs-1 in myeloma cell lines U266, RPMI8226. The expression of socs-1 gene mRNA in each myeloma cell line increased significantly after exposure to As(2)O(3) for 72 hours as compared with the cell lines of wild type (p < 0.05). And cell proliferation was significantly inhibited, both early apoptosis and later apoptosis ratios increased in dose-dependent manner. It is concluded that As(2)O(3) may induce socs-1 demethylation and up-regulate the expression of the gene. This study provides a new thought and direction for exploring possible mechanism of cell apoptosis induced by As(2)O(3) and multiple myeloma treatment by As(2)O(3).
