Differentiation of bone marrow derived from mesenchymal stem cells into cardiomyocyte-like cells induced by co-culture with rat myocardial cells.
- Author:
Rong-Li ZHANG
1
;
Er-Lie JIANG
;
Mei WANG
;
Zheng ZHOU
;
Wen-Jing ZHAI
;
Wei-Hua ZHAI
;
Hua WANG
;
Zhi-Yong WANG
;
Yu-Shi BAO
;
Hong DU
;
Ming-Zhe HAN
Author Information
1. Chinese Academy of Medical Sciences, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
cytology;
Cell Differentiation;
Cells, Cultured;
Coculture Techniques;
Mesenchymal Stromal Cells;
cytology;
Myocytes, Cardiac;
cytology;
Rats;
Rats, Wistar
- From:
Journal of Experimental Hematology
2008;16(5):1111-1115
- CountryChina
- Language:Chinese
-
Abstract:
The study was purposed to investigate the differentiation ability of mesenchymal stem cells (MSCs) into myocardial cells in vitro. Rat bone marrow-derived MSCs were labeled and co-cultured with neonatal rat cardiomyocytes (CM) for 5 - 7 days. The expression of cell surface antigens was detected by flow cytometry, and the expression of muscle-specific marker myosin and troponin T in labeled cells was detected by immunofluorescence. The results showed that in vitro cultured MSCs expressed CD90, CD44, CD105, CD54, not expressed CD34, CD45, CD31. After co-cultured with neonatal rat CM, labeled MSCs differentiated into cardiomyocyte-like cells expressing myosin and troponin T. It is concluded that MSCs can differentiate into cardiomyocyte-like cells when co-cultured with neonatal myocardial cells in vitro. In co-culture of two kind of cells in ratio of four to one showed obvious efficacy differentiating MSCs into CMs.
