GFP used as a reporter system for optimization of K562 cell transfection.
- Author:
Li-Ping DOU
1
;
Jun-Hua LIU
;
Chang WANG
;
Jing-Hua LIU
;
Hui-Yuan KANG
;
Yu JING
;
Yu ZHAO
;
Jian BO
;
Quan-Shun WANG
;
Fang-Ding LOU
;
Li YU
Author Information
1. Department of Hematology, PLA General Hospital, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Electroporation;
Genes, Reporter;
Green Fluorescent Proteins;
genetics;
Humans;
K562 Cells;
Liposomes;
Transfection
- From:
Journal of Experimental Hematology
2008;16(5):1174-1176
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to obtain higher efficiency in gene transfection into K562 cells and to study the role of green fluorescence protein (GFP) as a reporter system. Transfection efficiencies with different methods including electroporation and lipofectamine 2000 transfection, were observed and calculated under fluorescent microscopy by using GFP as a reporter system. The results showed that the transfection efficiency with electroporation (10%) was higher than that with lipofectamine 2000 (1%). In conclusion, the electroporation is a more ideal method for introduction of foreign gene into K562 cells. GFP can be used as a reporter system for optimizing transfection of K562 cells.
