Cytokine contents in single donor platelets during storage.
- Author:
Jian XU
1
;
Zhuo-Lan SHEN
;
Li YU
;
Jin YANG
;
Ron YU
;
Zhong-Hua MEN
;
Hang-Jun LU
;
Li-Xing YAN
Author Information
1. Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
- Publication Type:Journal Article
- MeSH:
Blood Platelets;
metabolism;
CD40 Ligand;
blood;
Cell Separation;
methods;
Chemokine CCL5;
blood;
Cytokines;
blood;
Humans;
Interleukin-8;
blood;
Platelet Count;
Specimen Handling;
Transforming Growth Factor beta;
blood;
Vascular Endothelial Growth Factor A;
blood
- From:
Journal of Experimental Hematology
2008;16(5):1185-1187
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the changes of cytokine contents in single donor platelets (SDPs) collected by using MCS(+), Trima, Amicus blood cell separators during storage. 18 portions of SDPs were collected by MCS(+), Trima, Amicus blood cell separators, were preserved in standard condition of blood bank, the levels of cytokines such as IL-8, RANTES, CD154, TGF-beta(1) and VEGF were detected by ELISA at 1, 3, 5, 7 days during storage. The results showed that the levels of IL-8, RANTES, CD154, TGF-beta(1) and VEGF in SDPs collected by blood cell separators MCS(+), Trima, and Amicus gradually increased with prolonging of time during storage, but the increase of IL-8 level in SDPs collected by MCS(+) separator was significant difference from SDPs collected by Trime and Amicus separators (p < 0.05). It is concluded that the all collected SDPs mentioned above express IL-8, RANTES, CD154, TGF-beta(1) and VEGF during storage, and their cytokine levels show a tendency to increase with prolonging of time during storage, apheresis platelets with less leukocytes express IL-8 lower.
