Expression of erythropoietin receptor in leukemia cells and relation of erythropoietin level with leukemic anemia.
- Author:
Mei FENG
1
;
Yu-Cui LI
Author Information
1. Department of Hematology, Shanxi People Hospital, Taiyuan 030012, Shanxi Province, China. tyfengmei@vip.sohu.com
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Anemia;
blood;
etiology;
metabolism;
Case-Control Studies;
Child;
Child, Preschool;
Erythropoietin;
blood;
Female;
Humans;
Leukemia, Myeloid, Acute;
Male;
Middle Aged;
Receptors, Erythropoietin;
metabolism;
Recombinant Proteins;
Young Adult
- From:
Journal of Experimental Hematology
2008;16(6):1265-1270
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the expression of erythropoietin receptor (EPOR) in leukemic cells and the relationship of serum erythropoietin level with anemia in acute leukemia patients, so as to provide a new theoretical basis for the cytokine therapy in acute leukemia with anemia. The EPOR in 30 AL patients was detected by using reverse transcription polymerase chain reaction (RT-PCR), the level of serum erythropoietin was detected by chemiluminescence analysis, the hemoglobin level was assayed by automatic blood counting instrument. The results indicated that EPOR was expressed in 18 out of 30 AL patients, the expression rate of EPOR in AL patients was 60%, however, but the EPOR expression rate in AML was 61.9% (13/21) and 55.6% (5/9) in ALL, the EPOR expression rate was no significant difference between AML and ALL. The EPOR expression rate was significantly lower than that in control group (86.7%) (p<0.05). The relative level of EPOR expression in AML was higher than that in ALL (p<0.05), the average level of EPOR expression in AL was significantly lower than that in control group (p<0.01). The level of sEPO in 30 AL patients was significantly higher than that in control group (p<0.01), and there was negative correlation between the levels of sEPO and Hb (p<0.01). It is concluded that the EPOR is expressed in cells of AL, but the expressive level is low. The EPOR expression rate shows no significant difference between AML and ALL. The mechanism of negative feedback to anemia in acute leukemia is intact. Anemia of acute leukemia is not completely associated with inadequate erythropoietin production and relates to hemopoiesis defect that considered as the main reason. Recombinant human erythropoietin is widely used in treatment of anemia caused by acute leukemia. Whether the treatment with rh-EPO for acute leukemia with anemia will enhance the proliferation of leukemia cells, this problem should be explored further.
