Expression of G-CSF and GM-CSF receptors on CD34 positive cells in aplastic anemia and myelodysplastic syndrome patients and its significance.
- Author:
Hong-Zhi XU
1
;
Ai LI
;
Yuan YU
;
Jian-Feng LI
;
Xin LIU
;
Xiang-Hua WANG
;
Xin WANG
;
Gong-Li XU
Author Information
1. Department of Hematology, Provincial Hospital, Shandong University, Jinan 250021, Shandong Province, China. xuhongzhi1965@sina.com
- Publication Type:Journal Article
- MeSH:
Adult;
Anemia, Aplastic;
metabolism;
Antigens, CD34;
immunology;
Bone Marrow Cells;
cytology;
immunology;
metabolism;
Case-Control Studies;
Female;
Flow Cytometry;
Humans;
Male;
Middle Aged;
Myelodysplastic Syndromes;
metabolism;
Receptors, Granulocyte Colony-Stimulating Factor;
metabolism;
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor;
metabolism
- From:
Journal of Experimental Hematology
2008;16(6):1308-1311
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to detect the ratio of CD34+ cells in bone marrow mononuclear cells (BMMNCs) and the expression rate of G(M)-CSFR on CD34+ cells in bone marrow of the patients with aplastic anemia (AA) and myelodysplastic syndrome (MDS). The ratio of CD34+ cells in BMMNCs and the expression rate of G(M)-CSFR on cells of 27 AA patients, 45 MDS patients and 20 controls were detected by flow cytometry (FCM). The results showed that the ratio of CD34+ cells in BMMNCs of AA patients reduced and was significantly different from controls (p<0.05), the ratio of CD34+ cells in MDS patients elevated and was significantly different from controls (p<0.05). Compared with controls and MDS-RA patients, the ratio of CD34+ cells in MDS-RAEB patients significantly elevated (p<0.05), but there was no significant difference between MDS-RA patients and controls (p>0.05). The ratio of CD34+ cells in MDS-RA patients was significantly higher than that in AA patients (p<0.05). There was no significant difference in expression rate of G-CSFR on CD34+ cells between AA patients and controls, MDS patients and controls, AA patients and MDS patients, MDS-RA patients and MDS-RAEB patients (p>0.05). The expression rate of GM-CSFR in MDS patients was significantly higher than that in AA patients and controls (p<0.05), but there was no significant difference between AA patients and controls, MDS-RA patients and MDS-RAEB patients (p>0.05). In AA patients, the ratio of CD34+ cells in BMMNCs was less than 0.1% accounts for 6/8 SAA patients, compared with 2/19 in CAA (p<0.05). There was no correlation between the expression rate of either G-CSFR or GM-CSFR and neutrophil count at diagnosis (r=0.058 and r=0.044). In MDS patients, there was no correlation between bone marrow CD34+ cells ratio and peripheral neutrophil count at diagnosis (r=-0.335). And there was no correlation between the expression of either G-CSFR or GM-CSFR and neutrophil count on diagnosis (r=0.064 and r=0.051). It is concluded the detection of CD34+ cells and their surface expression rate of G(M)-CSFR in AA and MDS is useful in diagnosis and differential diagnosis of these two diseases.
