Mesenchymal stem cells derived from human umbilical cord tissue modulate the secretion of antiplatelet antibody from splenocytes of ITP patients in vitro.
- Author:
Zhi-Yong QIU
1
;
Shao-Guang YANG
;
Zhen-Ping CHEN
;
Qin-Jun ZHAO
;
Xiao-Li CHEN
;
Ze-Ping ZHOU
;
Ren-Chi YANG
;
Zhong-Chao HAN
Author Information
1. Institute of Hematology, Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300020, China.
- Publication Type:Journal Article
- MeSH:
Antibodies;
metabolism;
Blood Platelets;
immunology;
CD4-Positive T-Lymphocytes;
cytology;
Cell Proliferation;
Humans;
Infant, Newborn;
Lymphocyte Activation;
Mesenchymal Stromal Cells;
physiology;
Purpura, Thrombocytopenic, Idiopathic;
metabolism;
Spleen;
cytology;
Umbilical Cord;
physiology
- From:
Journal of Experimental Hematology
2008;16(6):1372-1375
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to investigate the potential immunotherapeutical values of umbilical cord tissue-derived mesenchymal stem cells (UC-MSC) on patients with chronic idiopathic thrombocytopenic purpura (ITP). UC-MSC was cocultured in vitro with splenocytes isolated from ITP patients who experienced splenectomy. The level of IgG antiplatelet antibody (PAIgG) was determined by a competitive micro-enzyme-linked immunosorbent assay (ELISA) method. The proliferation of platelet-reactive CD4+ T lymphocytes was also measured in the presence of UC-MSCs. The results showed that UC-MSCs could stimulate the spontaneous secretion of PAIgG in supernatants; In the platelet-inducing condition, UC-MSC inhibited the production of PAIgG at a low ratio of 1 UC-MSC to 100 splenocytes, but promoted at a high proportion of 1 UC-MSC to 10 splenocytes. Moreover, UC-MSC exerted a suppressive effect on proliferation of platelet-reactive T helper cells in a dose-dependent manner. It is concluded that the UC-MSCs can regulate secretion of antiplatelet antibodies in vitro. Its concrete regulation mechanism and potential immunotherapeutical value are need to further study.
