Effect of total body irradiation on cellular senescence related indexes of bone marrow mesenchymal stem cells.
- Author:
Jie MA
1
;
Hong-Lan WANG
;
Jing LI
;
Ming-Xia SHI
;
Bing-Zong LI
;
Bin CHEN
;
Jian-Li HU
;
Chun-Hua ZHAO
;
Hui SUN
Author Information
1. Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
cytology;
radiation effects;
Cells, Cultured;
Cellular Senescence;
radiation effects;
Male;
Mesenchymal Stromal Cells;
cytology;
radiation effects;
Mice;
Mice, Inbred C57BL;
Whole-Body Irradiation
- From:
Journal of Experimental Hematology
2008;16(6):1387-1391
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to explore the changes in cellular senescence related indexes of bone marrow mesenchymal stem cells (BMMSCs) after total body irradiation (TBI). At different time points after 4 Gy irradiation, BMMSCs were isolated from male C57BL/6 mice and cultured. Morphology, senescence-associated beta-galactosidase (SA-beta-gal) staining and cell cycle analysis were used to evaluate the changes in BMMSCs at cellular level while real-time RT-PCR was used to detect the alterations in senescence related gene expression including p16INK4a, p21Cip1/Waf1, p53 and TGF-beta1. The results showed that within 4 weeks after exposure to 4 Gy TBI, the morphology of BMMSCs and the expression level of SA-beta-gal were not significantly changed, the cellular senescence-related cell cycle arrest was not occurred and the senescence related gene expression level was not increased. It is concluded that at the early stage after 4 Gy TBI, the related molecular level of cellular senescence in BMMSCs is not changed.
