Changes in cyclooxygenase gene expression during spontaneous recovery from stress ulcer in rats.
- Author:
Jun XU
1
;
Yu-gang SONG
;
Xian-fu SANG
;
Guang-xin BAO
;
Xu LI
;
Gang WU
;
Dong-shen CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cyclooxygenase 1; biosynthesis; genetics; Cyclooxygenase 2; biosynthesis; genetics; Gastric Mucosa; enzymology; Male; Prostaglandin-Endoperoxide Synthases; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Sprague-Dawley; Remission, Spontaneous; Stomach Ulcer; enzymology; Stress, Physiological; complications
- From: Journal of Southern Medical University 2006;26(1):91-97
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the changes in gene expression of cyclooxygenase (COX) during spontaneous recovery from stress ulcer in rats exposed to water immersion and restraint stress (WRS).
METHODSA rat model of stress ulcer was established by means of WRS, in which the changes in COX expression were detected with immunohistochemistry and reverse transcription (RT)-PCR.
RESULTSVery low levels of COX-2 expression were detected in the gastric mucosa of the control rats, and the expression increased significantly during the healing process of the stress ulcer (P<0.05). COX-1 expression in the gastric mucosa showed no significant difference between the control group and the stress ulcer groups during healing (P>0.05).
CONCLUSIONCOX-1 and COX-2 expressions in rat gastric mucosa during the recovery from stress ulcer participate in the recovery of the damaged mucosa possibly by mediating prostaglandin secretion.
