Preliminary identification and characterization of in vitro cultured bone marrow stromal cells for their bioactivity and osteogenic potential.
- Author:
Xiao-li TU
1
;
Hong-wei LIU
;
Cai-liang LI
;
Xiao-hua REN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; Cell Differentiation; physiology; Cells, Cultured; Dogs; Male; Osteoblasts; cytology; Osteogenesis; Stromal Cells; cytology
- From: Journal of Southern Medical University 2006;26(1):111-113
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo induce the differentiation of bone marrow stromall cells (BMSCs) isolated from Beagles into osteoblasts in vitro and identify the osteogenic potential and bioactivity of the BMSCs.
METHODSPrimary cultured BMSCs isolated from Beagles were subcultured in mineralization medium to induce their differentiation into osteoblasts, whose morphological characteristics and proliferation status were observed by phase-contrast microscope. The osteogenic activity of the cells was evaluated with von Kossa staining of the mineralized nodules and determination of the alkaline phosphatase activity.
RESULTBMSCs cultured in vitro showed obvious osteogenic capacity in DMEM. Von Kossa staining of the mineralized nodules and alkaline phosphatase detection of the passaged cells both yielded positive results.
CONCLUSIONBMSCs cultured in vitro contain osteogenic precursor cells, and the passaged cells possess osteogenic potential.
