Screening differentially expressed genes in denucleated K562 cells with restriction display technique.
- Author:
Min WEI
1
;
Wen-li MA
;
Yan-bin SONG
;
Xiang-ming MAO
;
Ling LI
;
Wen-ling ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Aquaporin 1; biosynthesis; genetics; Cytochalasin B; pharmacology; Electrophoresis, Polyacrylamide Gel; Gene Expression Profiling; methods; Gene Expression Regulation, Neoplastic; drug effects; Humans; K562 Cells; Oligonucleotide Array Sequence Analysis; Reverse Transcriptase Polymerase Chain Reaction
- From: Journal of Southern Medical University 2006;26(2):162-165
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen differentially expressed genes in cytochalasin B (CB)-induced denucleated K562 cells by restriction display (RD) technique.
METHODSThe total RNA was isolated and purified from K562 cells before and after CB (10 mug/ml) treatment. The mRNA from both treated and untreated K562 cells were reversely transcribed into cDNA, and the differentially expressed genes were separated using RD technique combined with polyacrylamide gel electrophoresis and sliver staining, followed by cloning, sequencing and homology analysis against GenBank database of these genes.
RESULTSSeven differentially expressed genes were identified in CB-treated cells including aquaporin 1 (AQP1) gene, which was verified to be up-regulated after CB treatment by RT-PCR.
CONCLUSIONAQP1 gene might be in close association with the regulation of denucleation processes and CB-induced proliferation inhibition of K562 cells.
