Screening of Urine Culture Specimens by Gram Stain, Urinalysis and Urine Microscopic Examinations.
- Author:
Chul Hun CHANG
;
Tae Hee PARK
;
Yoon Seong JEONG
;
Hyung Hoi KIM
;
Weon Joo HWANG
- Publication Type:Original Article
- Keywords:
Gram stain;
Urine;
10-well slide galss;
Screening;
Bacteriuria
- MeSH:
Bacteriuria;
Coloring Agents;
Cost Savings;
Leukocytes;
Mass Screening*;
Pyuria;
Urinalysis*
- From:Korean Journal of Clinical Microbiology
2000;3(1):53-56
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The purpose of this study was to discover ways to screen urine culture specimens through Gram stains, urine stick analyses and microscopic examinations for the laboratory cost saving. METHODS: One hundred and fifty-eight urine specimens for culture were included. Fifty uL of urine were inoculated onto one well each of 10-well slide, dried on the hot plate, and Gram-stained. The results combined with routine urinalyses including urine nitrite and leukocyte esterase, and pyuria, were compared with the routine culture results. RESULTS: The screening of bacteriuria by Gram stains, urinalyses and microscopic examinations revealed the high sensitivity (91.9%) and negative predictive value(95.5%) with cost saving of 41.8% of inoculating media. Not considering the Gram stains, the screening revealed 83.8% sensitivity and 92.5% negative predictive value, even if the cost saving of inoculating media were as high as 50.1%. CONCLUSION: It was demonstrated that it was sensitive and economic and produced rapid preliminary results to screen bacteriuria by the Gram stains combined with urinalyses and microscopic examinations.
