Particulate Matter 10 from Asian Dust Storms Induces the Expression of Reactive Oxygen Species, NF-kappaB, TGF-beta and Fibronectin in WI-26 VA4 Epithelial Cells.
10.4046/trd.2008.65.6.504
- Author:
Kyeong Seon PARK
1
;
Yu Jin KIM
;
Jin Young YOON
;
Sun Young KYUNG
;
Chang Hyeok AN
;
Sang Pyo LEE
;
Jeong Woong PARK
;
Sung Hwan JEONG
Author Information
1. Gachon University of Medicine and Science, Korea.
- Publication Type:Original Article
- Keywords:
Particulate matter;
Asian dust;
Reactive oxygen species;
Pulmonary fibrosis
- MeSH:
Asian Continental Ancestry Group;
Blotting, Western;
Dust;
Epithelial Cells;
Fibronectins;
Humans;
NF-kappa B;
Particulate Matter;
Pulmonary Fibrosis;
Reactive Oxygen Species;
Transforming Growth Factor beta
- From:Tuberculosis and Respiratory Diseases
2008;65(6):504-511
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Particulate matter may be toxic to human tissue. Ambient air particulate matter < or =10micrometer in aerodynamic size (PM10), which changes under different environmental conditions, is a complex mixture of organic and inorganic compounds. The Asian dust event caused by meteorological phenomena can also spread unique particulate matter in affected areas. We evaluated production of ROS, TGF-beta, fibronectin, and NF kappa B by exposing normal epithelial cells to Asian dust particulate matter. METHODS: Bronchial epithelial cells were exposed to 0, 50, 100microgramg/ml of a suspension of PM10 for 24 h. ROS were detected by measurement of DCF release from DCF-DA by FACScan. TGF-beta, fibronectin, and NF kappa B were detected by western blotting. RESULTS: PM10 exposure increased the expression of TGF-beta, fibronectin, and NF kappa B. ROS production and TGF-betalevels were significantly higher with 50 or 100microgram/ml PM10. Fibronectin and NF kappa B production were significantly higher after 100microgram/ml of PM10. CONCLUSION: PM10 from Asian dust particles might have fibrotic potential in bronchial epithelial cells via ROS induction after PM10 exposure.
