Development of an immunochromayography assay method for the detection of Yersinia pestis.
- Author:
Hong ZHU
1
;
Chun-hua ZHANG
;
Hua TAN
;
Jun HE
;
Bin ZHAO
;
Qing DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Proteins; analysis; immunology; Immunoassay; methods; Sensitivity and Specificity; Yersinia pestis; immunology
- From: Chinese Journal of Epidemiology 2006;27(3):253-255
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method of immunochromatography assay (ICA) with sensitive, specific, rapid, simple and suitable for the detection of Yersinia pestis antigen at the local laboratories.
METHODSColloidal gold labeled with the anti-F1 antibody of Yersinia pestis, was connnected with the anti-F1 antibody of Yersinia pestis to pyroxylin membrane and assembled them to the dipstick of ICA.
RESULTSResults showed that the rates of sensitivity for F1 antigen and Yersinia pestis were 1 ng/ml and 1.56 x 10(5) CFU/ml respectively. However, Yersinia pseudotuberculosis et al could not be detected by dipstick of ICA.
CONCLUSIONThe method of ICA appeared to be consistent to those of r-IHA with better specificity and sensitivity but was simple and rapid for the detection of Yersinia pestis and F1 antigen.
