Primary investigation on variable but nonculturable-state of enterotoxigenic Escherichia coli in vitro.
- Author:
Dao-li CHEN
1
;
Yu-hua FENG
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Bacterial; Drug Resistance, Bacterial; Enterotoxigenic Escherichia coli; drug effects; immunology; pathogenicity; Microbiological Techniques
- From:Chinese Journal of Epidemiology 2006;27(5):409-411
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE7 variable but nonculturable-state strains of Enterotoxigenic Escherichia coli (ETEC) during the routine bacterial subculture were found in our lab and their morphology and antigen studied. Biological features, antigens and pathogenicity of the revertants were also tested and compared to that of the initial strains in order to detect their variations.
METHODSBiological variations between the variable but nonculturable-state and the revertant of every strain were detected, using the routine gram-staining, reverting the isolates in animal intestinal, reverting their pathogenicity, serological agglutination, biochemical identifications and antibiotic resistance tests.
RESULTSFor the 7 variable but nonculturable-state strains of ETEC,other than the trains that had changed into sphero vegetale cells, there were no other obvious variations found. However, high pathogenicity of these strains still remained.
CONCLUSIONThe presence of variable but nonculturable-state strains suggested that the routine method of bacteria storage should be changed and more attention should be paid to realize the existence of this kind of bacteria during the routine surveillance of the communicable diseases.