Study on fluoroquinolone resistance and the relationship between resistance and mutations of gyrA and parC in Neisseria gonorrhoeae.
- Author:
Jin-shui XU
1
;
Bei WANG
;
Chang-xian WANG
;
Hai-jian GOU
;
Zu-huang MI
;
Jian-hua LING
Author Information
- Publication Type:Journal Article
- MeSH: China; DNA Gyrase; genetics; DNA Topoisomerase IV; genetics; Drug Resistance, Bacterial; Fluoroquinolones; pharmacology; Genotype; Gonorrhea; drug therapy; Humans; Neisseria gonorrhoeae; drug effects; genetics; isolation & purification; Phenotype
- From: Chinese Journal of Epidemiology 2006;27(8):702-704
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the phenotypic and genotypic resistance to Fluoroquinolones in Neisseria gonorrhoeae (NG) isolated in Jiangsu province of China.
METHODSIn-vitro, susceptibility testing of ciprofloxacin and levofloxacin against ninety-five clinical isolates were determined by agar dilution method. Detection of mutation in the gyrA and parC genes was performed by polymerase chain reaction (PCR) assay and sequence analysis.
RESULTSThe clinical isolates demonstrated 100% resistance to ciprofloxacin. Based on gyrA and parC mutations, 18 types could be categorized among the 54 isolates. Based on the same gyrA mutations,isolates with high MIC appeared to have had more mutations in parC gene.
CONCLUSIONThe status of resistance to ciprofloxacin in NG was quite serious, and ciprofloxacin treatment for the treatment of NG infections in Jiangsu province should not be recommended. The results from this study suggested that mutations in the parC gene had contributed to the development of high Fluoroquinolone resistance in NG.
