Effects of sildenafil citrate on mice hearing
10.3760/cma.j.issn.1673-0860.2014.06.009
- VernacularTitle:枸橼酸西地那非对小鼠听觉的影响
- Author:
Xiaoqin LUO
1
;
Xuyao GUO
;
Lin CHEN
;
Xiaohong CHEN
;
Xueyuan ZHANG
;
Wei YUAN
Author Information
1. 第三军医大学附属西南医院耳鼻咽喉头颈外科
- Keywords:
Sildenafil citrate;
Evoked potentials,auditory,brain stem;
Hair cells;
Mice
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2014;49(6):483-487
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of this investigation was to study the effects of the Sildenafil citrate on mice hearing.Methods Seven-week-old adult male Kunming mice were used.The mice were randomly divided into four groups with 10 mice in each group.Sildenafil groups were orally administered daily with sildenafil [0.1 mg/(kg · d),1 mg/(kg · d),10 mg/(kg · d)] and control group was orally administered with normal saline.Then mice were tested for auditory brainstem response(ABR) to observe the changes of ABR's thresholds at before administration and 1,5,10,15,20 day afterwards.The mice basilar membrane samples were studied by immunofluorescent labeling.High performance liquid chromatography was used for determination the concentration of sidenafil in endolymph of mice cochlea.Statistical analysis was performed using SPSS 13.0.Results After 30 min following administration,the Sildenafil in endolymph of mice cochlear could be assayed by high performance liquid chromatography,and it was doserelated.Sildenafil increased the hearing thresholds with the time of administration.Hearing thresholds increased significantly in the sidenafil group at 20 d compared to the control group (P ＜ 0.05).After administered high dose of Sildenafil,on the 20th day,the ABR thresholds average threshold was (60.0 ± 10.0) dBnHL,and the control group was (14.5 ± 6.0) dBnHL.Hair cells damages in the base ring of cochlea could be observed in experimental group in a concentration-dependent manner.Conclusion Sidenafil can pass through blood-labyrinth barrier to the inner ear,and doses of sildenafil administration can induce hearing impairment in mice.
