Inhibition of Th2 reaction by thymic stromal lymphopoietin blockade in vitro.
- Author:
Liting WEN
1
;
Xu LI
;
Li QIAO
;
Wenjuan MI
;
Ye WANG
;
Zhaohui SHI
;
Jianhua QIU
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Transdifferentiation; Cells, Cultured; Coculture Techniques; Cytokines; antagonists & inhibitors; Dendritic Cells; drug effects; Mice; Mice, Inbred BALB C; Th2 Cells; cytology
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2014;49(6):488-492
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of thymic stromal lymphopoietin (TSLP) on transformation of dendritic cell (DC) and T cell in vitro.
METHODSMouse-derived immature dendritic cells and T lymphocytes were co-cultured in vitro, which were divided into 4 groups (TSLP stimulation group, TSLP stimulation and its receptor blocking group, ovalbumin stimulation group and ovalbumin stimulation and TSLP receptor blocking group). IL-4, IL-8 and IFN-β in cell culture supernatant were detected after 2 days by ELISA. SPSS 13.0 software was used to analyze the data.
RESULTSIL-4 levels of TSLP receptor blocking groups [(48.84 ± 1.56) pg/ml, (52.53 ± 2.36) pg/ml]were significantly lower than those of corresponding TSLP stimulation group and ovalbumin stimulation group [(72.55 ± 7.76) pg/ml, (80.47 ± 21.93) pg/ml;t = 5.994, P < 0.05;t = 2.534, P < 0.05]. However, there were not significant differences of IL-8 and IFN-β expression between corresponding two groups of whether or not TSLP receptor blocking (all P > 0.05).
CONCLUSIONTSLP receptor blockade in vitro can inhibit T lymphocyte transformation to Th2, which may provide a new therapeutic strategy for clinical Th2 dominant diseases such as allergic rhinitis and asthma.
