Inhibition of Th2 reaction by thymic stromal lymphopoietin blockade in vitro
10.3760/cma.j.issn.1673-0860.2014.06.010
- VernacularTitle:阻断胸腺基质淋巴细胞生成素抑制Th2反应的体外研究
- Author:
Liting WEN
1
;
Xu LI
;
Li QIAO
;
Wenjuan MI
;
Ye WANG
;
Zhaohui SHI
;
Jianhua QIU
Author Information
1. 第四军医大学西京医院耳鼻咽喉头颈外科
- Keywords:
Cytokines;
Dendritic cells;
T-lymphocytes;
Th2 cells;
Immunoglobulins;
Receptors,cytokines;
Cell,cultured
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2014;49(6):488-492
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of thymic stromal lymphopoietin (TSLP) on transformation of dendritic cell (DC) and T cell in vitro.Methods Mouse-derived immature dendritic cells and T lymphocytes were co-cultured in vitro,which were divided into 4 groups (TSLP stimulation group,TSLP stimulation and its receptor blocking group,ovalbumin stimulation group and ovalbumin stimulation and TSLP receptor blocking group).IL-4,IL-8 and IFN-β in cell culture supernatant were detected after 2 days by ELISA.SPSS 13.0 software was used to analyze the data.Results IL-4 levels of TSLP receptor blocking groups [(48.84 ± 1.56) pg/ml,(52.53 ± 2.36) pg/ml] were significantly lower than those of corresponding TSLP stimulation group and ovalbumin stimulation group [(72.55 ± 7.76) pg/ml,(80.47 ± 21.93) pg/ml ; t =5.994,P ＜ 0.05 ; t =2.534,P ＜ 0.05].However,there were not significant differences of IL-8 and IFN-β expression between corresponding two groups of whether or not TSLP receptor blocking (all P ＞ 0.05).Conclusion TSLP receptor blockade in vitro can inhibit T lymphocyte transformation to Th2,which may provide a new therapeutic strategy for clinical Th2 dominant diseases such as allergic rhinitis and asthma.
