Study on the induced differentiation of induced pluripotent stem cells into cochlear hair cell-like cells and spiral ganglion neuron-like cells in vitro.
- Author:
Lina GUAN
1
;
Yanhong CHEN
;
Hengtao ZHU
;
Jing CHEN
;
Hongqun JIANG
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; Cochlea; physiology; Coculture Techniques; Hair; Hair Cells, Auditory; In Vitro Techniques; Induced Pluripotent Stem Cells; Mice; Mice, Inbred ICR; Neurons; Spiral Ganglion; physiology
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2014;49(8):680-686
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn this study, we investigated the potential of mouse induced pluripotent stem cells (iPSC) for use as a source of transplants for the restoration of auditory hair cells and spiral ganglion neurons.
METHODSWe co-cultured the mouse iPSC with the cells of the cochlear organ of Corti or the modiolus in vitro. The cochlear organ of Corti (which contains cochlear hair cells) and the modiolus (which contains auditory spiral ganglion neurons) were obtained from postnatal day 3 (P3) CD-1 ICR mice. After 18 days of coculture with the cells of newborn mouse cochleae. The expressions of hair cell markers (Myosin VIIa, Math1, Calretinin, Espin) and Spiral ganglion neuron markers [Nestin, Neurofilament-M, β-III Tubulin, Vesicular glutamate transporter 1(VGluT1)] were detected by immunocytochemical analysis.
RESULTSImmunocytochemical analysis results indicated that the differentiated iPSC expressed auditory hair cell markers (MyosinVIIa,Math1, Calretinin, Espin ) and spiral ganglion markers (Nestin, Neurofilament-M,β-III Tubulin,VGluT1).
CONCLUSIONMouse iPSC in virto cultured could successfully be induced to differentiate into hair cell-like cells and spiral ganglion-like cells with hair cell and spiral ganglion molecular markers.
