Study on the induced differentiation of induced pluripotent stem cells into cochlear hair cell-like cells and spiral ganglion neuron-like cells in vitro
10.3760/cma.j.issn.1673-0860.2014.08.012
- VernacularTitle:小鼠诱导性多潜能干细胞向内耳毛细胞和螺旋神经节细胞分化的体外研究
- Author:
Lina GUAN
1
;
Yanhong CHEN
;
Hengtao ZHU
;
Jing CHEN
;
Hongqun JIANG
Author Information
1. 南昌大学第一附属医院耳鼻咽喉头颈外科
- Keywords:
Induced pluripotent stem cells;
Organ of Corti;
Hair cells;
Spiral ganglion;
Cell differentiation;
Mice
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2014;49(8):680-686
- CountryChina
- Language:Chinese
-
Abstract:
Objective In this study,we investigated the potential of mouse induced pluripotent stem cells(iPSC) for use as a source of transplants for the restoration of auditory hair cells and spiral ganglion neurons.Methods We co-cultured the mouse iPSC with the cells of the cochlear organ of Corti or the modiolus in vitro.The cochlear organ of Corti (which contains cochlear hair cells) and the modiolus (which contains auditory spiral ganglion neurons) were obtained from postnatal day 3 (P3) CD-1 ICR mice.After 18 days of coculture with the cells of newborn mouse cochleae.The expressions of hair cell markers (Myosin Ⅶ a,Math1,Calretinin,Espin) and Spiral ganglion neuron markers [Nestin,Neurofilament-M,β-Ⅲ Tubulin,Vesicular glutamate transporter 1 (VGluT1)] were detected by immunocytochemical analysis.Results Immunocytochemical analysis results indicated that the differentiated iPSC expressed auditory hair cell markers (Myosin Ⅶ a,Math1,Calretinin,Espin) and spiral ganglion markers (Nestin,Neurofilament-M,β-Ⅲ Tubulin,VGluT1).Conclusion Mouse iPSC in virto cultured could successfully be induced to differentiate into hair cell-like cells and spiral ganglion-like cells with hair cell and spiral ganglion molecular markers.
