The nucleus origin of acoustically evoked short latency negative response in guinea pigs
10.3760/cma.j.issn.1673-0860.2014.10.012
- VernacularTitle:声诱发短潜伏期负电位豚鼠模型的神经核团定位
- Author:
Shaohua ZHANG
1
;
Wenqin HUANG
;
Chenxi LIN
;
Jin CHEN
;
Dongxiao NONG
;
Anzhou TANG
Author Information
1. 广西医科大学第一附属医院耳鼻咽喉头颈外科
- Keywords:
Evoked potentials,auditory;
Vestibular nucleus;
Cochlear nucleus;
Guinea pigs
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2014;49(10):848-853
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study established a model of acoustically evoked short latency negative response (ASNR) in guinea pigs.Stereotaxic coordinate guided electrolytic lesion was applied to animal brainstem nuclei,the vestibular nucleus and the cochlear nucleus,to define the neural origin of ASNR.Methods Twenty four guinea pigs with normal hearing were randomly divided into the control group (8 subjects,16 ears) and the deafened group (16 subjects,32 ears).Each animal experienced the auditory brainstem response (ABR) test.According to the presence of ASNR,the deafened group was further divided into ASNR group and non-ASNR group.Electrolytic lesion was conducted to the vestibular nucleus and cochlear nucleus respectively,followed by ABR test.The lesion structures were verified by brainstem slice and microscope.Results In deafened group,the ASNR was elicited in 10 ears (31.3％).The ASNR was eliminated due to the electrolytic destruction to the vestibular nucleus,but it remained unchanged after the same procedure to the cochlear nucleus.Conclusion It is clear that the ASNR is originated from the vestibular nucleus,but not the cochlear nucleus.
