Effect of angiotensin II type 1 receptor and angiotensin-converting enzyme gene silencing on nuclear factor-kappaB activity in hepatic stellate cells.
- Author:
Xu LI
1
;
Yi-Jun ZHANG
;
Ying MENG
;
Gao-Su ZHOU
;
Zhen-Shu ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Hepatic Stellate Cells; cytology; metabolism; Humans; NF-kappa B; genetics; metabolism; Peptidyl-Dipeptidase A; genetics; RNA Interference; RNA, Small Interfering; genetics; Receptor, Angiotensin, Type 1; genetics; Transfection
- From: Journal of Southern Medical University 2009;29(3):402-404
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of angiotensin II (AngII) type 1 (AT-1) receptor and angiotensin-converting enzyme (ACE) gene silencing on nuclear factor-kappaB (NF-kappaB) activity in hepatic stellate cells (HSCs).
METHODSpSilencer/AT-1 alpha receptor siRNA and pSilencer/ACE siRNA plasmids were transfected into cultured HSC-T6 cells, which were subsequently stimulated by 10(-6) mol/L AngII or ACE inhibitor (ACEI). The DNA binding activity of NF-kappaB in the transfected cells was analyzed using electrophoretic gel mobility shift assay (EMSA).
RESULTSs Gel shift studies showed that stimulation of the HSCs by AngII markedly increased the DNA-binding activity of NF-kappaB, which was inhibited by the transfection with pSilencer/ AT-1 alpha receptor siRNA plasmid or pSilencer/ACE siRNA plasmid.
CONCLUSIONAT-1 alpha receptor and ACE gene silencing result in inhibition of NF-kappaB activity in HSCs in vitro.
