Time course of let-7a expression in the cell cycle of human cervical carcinoma HeLa cells.
- Author:
Jue-Yu ZHOU
1
;
Wen-Li MA
;
Hai-Lang YU
;
Wei-Wei XIAO
;
Wen-Ling ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Cycle; genetics; Gene Expression Regulation, Neoplastic; physiology; HeLa Cells; Humans; MicroRNAs; genetics; metabolism
- From: Journal of Southern Medical University 2009;29(3):442-445
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the time course of let-7a microRNA expression in the cell cycle of HeLa cells.
METHODSHeLa cells were synchronized in G(1), S and G(2)/M phases using double-thymidine block, and the cell cycle phases were defined by flow cytometry. Real-time quantitative RT-PCR was used to examine the expression of let-7a in HeLa cells in different cell cycle phases.
RESULTSThe synchronization rates of G(1), S and G(2)/M phases were 84.81%, 83.65% and 77.69%, respectively. Let-7a was constitutively expressed throughout the cell cycle in HeLa cells, but the expression levels in G(1) and S phases were lower than those in G(2)/M phase.
CONCLUSIONSCell cycle can significantly influence the expression level of let-7a, which may provide new clues to the understanding of the cell cycle control mechanisms.
