Expression of ERK5 in multidrug-resistant hepatocellular carcinoma cell line.
- Author:
Feng YAN
1
;
Xiao-Min WANG
;
Chao PAN
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; metabolism; pathology; Doxorubicin; pharmacology; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Fluorouracil; pharmacology; Humans; Liver Neoplasms; metabolism; pathology; Mitogen-Activated Protein Kinase 7; genetics; metabolism; RNA, Messenger; genetics; metabolism; Tumor Cells, Cultured
- From: Journal of Southern Medical University 2009;29(3):483-486
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expression of extracellular-signal regulated protein kinase 5 (ERK5) in multidrug-resistant hepatocellular carcinoma cell lines hepG2/ADM and BEL-7402/5-FU and in the parental cell lines (hepG2 and BEL-7402), and study the role of ERK5 in multidrug resistance of hepatocellular carcinoma.
METHODSMTT assay was used to determine the multidrug resistance (MDR) of hepG2/ADM and BEL-7402/5-FU cells. Real-time PCR and Western blotting were used to detect the expression of ERK5 at mRNA and protein levels in the 4 cell lines.
RESULTSThe resistance indices to ADM, 5-FU, and CDDP was 12.34, 5.74, and 3.81 in hepG2/ADM cells, and the resistance indices to 5-FU, VCR, OHP, MTX, and ADM was 15.32, 10.08, 5. 85, 6.74 and 3.26 in BEL-7402 cells, respectively. Compared with those in hepG2 and BEL-7402 cells, the ERK5 gene was up-regulated at both mRNA and protein levels in HepG2/ADM cells, but down-regulated at the mRNA level and up-regulated at the protein level in BEL-7402/5-FU cells.
CONCLUSIONSERK5 is related to multidrug resistance in HepG2/ADM and BEL-7402/5-FU cells, and may provide new clues for reversing multidrug resistance of hepatocellular carcinoma cells.
