Effects of Ciwujia in inducing marrow stromal cell differentiation into neuron-like cells in vitro.
- Author:
Qin YANG
1
;
Jun YANG
;
Peng XIE
;
Zhi-Lei ZENG
;
Ao LI
;
Jun YANG
;
Xiao-Dong ZHANG
;
Yong-Mei LI
;
Fa-Jin LÜ
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; cytology; drug effects; Cell Differentiation; drug effects; Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Eleutherococcus; Female; Glial Fibrillary Acidic Protein; metabolism; Intermediate Filament Proteins; metabolism; Male; Nerve Tissue Proteins; metabolism; Nestin; Neurons; cytology; Plant Extracts; pharmacology; Rats; Rats, Wistar; Stromal Cells; cytology; drug effects; Tubulin; metabolism
- From: Journal of Southern Medical University 2009;29(3):487-490
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of the traditional Chinese herbal drug Ciwujia in inducing the differentiation of marrow stromal cells (MSCs) into neuron-like cells.
METHODSRat MSCs isolated from the whole bone marrow were amplified by adherent culture in vitro and induced to differentiate into neuron-like cells using serum-free DMEM/F12 containing Ciwujia. The protein and mRNA expressions of nestin, beta-Tubulin III and glial fibrillary acidic protein (GFAP) in the differentiated cells were detected by indirect immunofluorescence method, Western blotting and RT-PCR.
RESULTSThe third-passage MSCs showed positive expression rates for CD44 and CD54 beyond 90% with decreased CD14 expression rate to 2.37%. Induction by Ciwujia of the MSCs resulted in cell body shrinkage and protrusion of the cell processes resembling those of neurons. The differentiated cells were positive for nestin and beta-Tubulin III expression and negative for GFAP as shown by immunofluorescence assay, Western blotting and RT-PCR.
CONCLUSIONCiwujia can induce the differentiation of rat MSCs into neuron-like cells in vitro.
