Establishment and assessment of Caco-2 cell in vitro absorption model.
- Author:
Long-Ying ZHA
1
;
Hai-Ji LUO
;
Hong DENG
;
Xin-Wei CHU
Author Information
- Publication Type:Journal Article
- MeSH: Caco-2 Cells; Cell Culture Techniques; methods; Epithelial Cells; cytology; metabolism; Humans; Intestinal Absorption; Intestines; cytology
- From: Journal of Southern Medical University 2009;29(3):548-550
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish and assess the Caco-2 cell in vitro absorption model.
METHODSCaco-2 cells were cultured on the millipore filters fixed in Snapwell transport chamber. The cell morphology, transepithelial electrical resistance, mannitol efflux rate and alkaline phosphatase activities were monitored during culture.
RESULTSAfter 21 days of in vitro culture, formation of tight junction was observed between the cells. The transepithelial electrical resistance reach a relatively stable value of 620-/+47 Omega.cm(2), the mannitol efflux rate was lower than 0.3%.h(-1).cm(-2), and the alkaline phosphatase activity in the apical side was significantly higher than that in the basolateral side.
CONCLUSIONThe established Caco-2 cell model shows similar morphology to intestinal epithelial cells with formation of polarity, and can be used as an in vitro model for absorption studies.
