Determination of ferulic acid in Fuan oral liquid by high-performance capillary electrophoresis.
- Author:
Yi-Lei LI
1
;
Ping ZHENG
;
Yuan YAN
;
Fang YANG
Author Information
- Publication Type:Journal Article
- MeSH: Coumaric Acids; analysis; Drugs, Chinese Herbal; chemistry; Electrophoresis, Capillary; methods; Quality Control
- From: Journal of Southern Medical University 2009;29(3):551-552
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a quality control method of Fuan oral liquid.
METHODSHigh-performance capillary electrophoresis (HPCE) was used to determine the content of feruild acid in Herba taraxaci, and thin-layer chromatography (TLC) was performed to identify Herba taraxaci and Bupleurum chinense. The condition of HPCE was optimized with a fuse silica capillary tube (70 microm x 60 cm) and 20 mmol/L sodium tetraborate buffer (pH9.18) at a constant voltage of 12 kV and temperature at 25 degrees celsius;, with the detection wavelength at 313 nm.
RESULTSClear spots were displayed on TLC. The calibration curve was linear in the range of 8-40 microg/ml for ferulic acid (Y=360.5-207.4, r=0.9997, n=5). The average recovery rate exceeded 95% with a RSD<3% (n=3).
CONCLUSIONThis method is simple and specific with a good reproducibility for quality control of Fuan oral liquid.
