A simple and efficient method for establishing a mouse model of orthotopic MB49 bladder cancer.
- Author:
Zhong-kun LIANG
1
;
Lin ZHANG
;
Zhi-ming HU
;
Zhong CHEN
;
Xin HUANG
;
Xiang-hua SHI
;
Wan-long TAN
;
Ji-min GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Cell Transformation, Neoplastic; Disease Models, Animal; Female; Mice; Mice, Inbred C57BL; Mitomycin; pharmacology; Organ Size; drug effects; Urinary Bladder; drug effects; pathology; Urinary Bladder Neoplasms; pathology
- From: Journal of Southern Medical University 2009;29(4):627-630
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a simple and efficient method for establishing a mouse model of orthotopic superficial bladder cancer.
METHODSC57BL/6 mice were anesthetized with sodium pentobarbital and catheterized with modified IV catheter (24 G). The mice were intravesically pretreated with HCl and then with NaOH, and after washing the bladders with phosphate-buffered saline (PBS), 100 microl (1 x 10(7)) MB49 cells were infused and allowed to incubate in the bladder for 2 h followed intravesical mitomycin C (MMC) administration. The tumor formation rate, survival, gross hematuria, and bladder weight were determined as the outcome variables, and the pathology of the bladders was observed.
RESULTSInstillation of MB49 tumor cells resulted in a tumor formation rates of 100% in all the pretreated groups while 0% in the control group without pretreatment. MMC significantly reduced the bladder weight as compared to PBS.
CONCLUSIONWe have successfully established a stable, reproducible, and reliable orthotopic bladder cancer model in mice.
