Effect of Shuanghuanglian injection on cerebral nuclear factor-kappaB expression in mice with viral encephalitis.
- Author:
Ye TIAN
1
;
Zheng-li DI
;
Hui LEI
;
Ge-juan ZHANG
;
Hua-di LIANG
;
Hui-ling CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Brain; drug effects; metabolism; Drugs, Chinese Herbal; administration & dosage; pharmacology; therapeutic use; Encephalitis, Viral; drug therapy; genetics; metabolism; Gene Expression Regulation; drug effects; Injections; Male; Mice; Mice, Inbred BALB C; NF-kappa B; genetics; metabolism; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Journal of Southern Medical University 2009;29(4):676-679
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Shuanghuanglian injection on cerebral expression of nuclear factor-kappaB (NF-kappaB) in mice with viral encephalitis.
METHODSThe mice with experimental viral encephalitis received treatment with Shuanghuanglian injection at the dose of 0.2, 1.5, and 5 for 5, 10 or 20 consecutive days. The total RNA of the brain tissue was extracted to analyze the protein and mRNA expression of NF-kappaB using Western blotting and RT-PCR, respectively.
RESULTSCompared with the control group, the mice with experimental viral encephalitis showed significantly increased protein and mRNA expressions of NF-kappaB (P<0.01). Treatment with Shuanghuanglian injection at the doses of 0.2 and 1.5 mg/kg significantly lowered NF-kappaB protein and mRNA expressions in the brain of mice with viral encephalitis (P<0.05), and the effect was even more obvious at the dose of 5 mg/kg (P<0.01).
CONCLUSIONShuanghuanglian injection can reduce the expression of NF-kappaB in the brain of mice with viral encephalitis in a dose- and time-dependent manner.
