Bone marrow stem cell mobilization to the peripheral blood and tumor necrosis factor-alpha expression in ischemic myocardium in rabbit models of hibernating myocardium.
- Author:
Qing-bin ZHAO
1
;
Chao-feng SUN
;
Ai-qun MA
;
Chang-cong CUI
;
Yue WU
;
Yu-ling TIAN
;
Yan ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, CD34; metabolism; Bone Marrow Cells; cytology; Coronary Vessels; surgery; Disease Models, Animal; Gene Expression Regulation; Hematopoietic Stem Cell Mobilization; Hibernation; Ligation; Male; Myocardial Ischemia; metabolism; physiopathology; surgery; therapy; RNA, Messenger; genetics; metabolism; Rabbits; Tumor Necrosis Factor-alpha; genetics
- From: Journal of Southern Medical University 2009;29(4):711-713
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes in peripheral blood bone marrow stem cells and tumor necrosis factor-alpha gene expression in the ischemic myocardium in rabbit models of hibernating myocardium.
METHODSTwenty-four male Japanese white rabbits were randomized into 4 groups, including a sham-operated group and 3 model groups with hibernating myocardium induced by partial ligation of the left anterior descending coronary artery. The percentage of CD34-positive cells in the peripheral blood was evaluated by flow cytometry, and TNF-alpha mRNA expression in the ischemic myocardium was determined by real-time RT-PCR in the 3 model groups (at 3, 7, or 28 days after the operation) and in the sham-operated group.
RESULTSIn rabbits with partial ligation of the left anterior descending coronary artery, the percentage of CD34-positive cells in the peripheral blood and myocardial TNF-alpha mRNA expression were significantly increased at 3 and 7 days after the operation in comparison with those in the sham-operated group and those at 28 days postoperatively (P<0.01). No significant differences were found in the percentage of CD34 positive cells or myocardial TNF-alpha mRNA expression between the sham-operated group and the rabbits 28 days after the coronary artery ligation (P>0.05).
CONCLUSIONBone marrow stem cell can be mobilized into the peripheral blood in rabbit hibernating myocardium model possibly by increasing TNF-alpha gene expression in the ischemic myocardium.
