Determination of the concentrations of interleukin-18 and other cytokines in the synovial fluid in patients with osteoarthritis.
- Author:
Yong LI
1
;
Jian-ming JIANG
;
De-hong YANG
;
Feng-long WANG
;
Zhong-xuan MAO
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Dinoprostone; biosynthesis; Female; Humans; Interleukin-18; metabolism; Male; Middle Aged; Osteoarthritis; metabolism; Synovial Fluid; metabolism
- From: Journal of Southern Medical University 2009;29(4):729-731
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the concentrations of interleukin-18 (IL-18), IL-6, IL-8, and prostaglandin E2 (PGE2) in the synovial fluid in patients with osteoarthritis (OA), and explore the role of IL-18 in the pathogenesis of OA.
METHODSThe synovial fluid was collected from 30 patients with knee OA, and the concentrations of IL-18 and the other cytokines were measured using enzyme-linked immunosorbent assay (ELISA). A linear regression was performed between IL-18 and the other cytokines.
RESULTSThe average IL-18 and PGE2 concentrations were 220-/+304 pg/ml and 89-/+104 pg/ml in the synovial fluid, respectively, and the two cytokines showed a positive correlation in the synovial fluid (r=0.628, P=0.001). The IL-18 concentration was also correlated to the concentrations of IL-6 (1200-/+1587 pg/ml, n=22; r=0.590, P=0.008) and IL-8 (5190-/+6024 pg/ml, n=9; r=0.776, P=0.014).
CONCLUSIONIL-18 can promote PGE2 production, which causes cartilage degradation in OA, thus therapies targeting this cytokine may prove an effective approach to early OA treatment.
