Effect of angiotensin1-7 on alpha-smooth muscle actin protein expression in rat hepatic stellate cells.
- Author:
Song-song YING
1
;
Xu LI
;
Mao-liang HUANG
;
Ying MENG
;
Zhen-shu ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Actins; metabolism; Angiotensin I; pharmacology; Angiotensin II; pharmacology; Animals; Calcium; metabolism; Cell Line; Gene Expression Regulation; drug effects; Hepatic Stellate Cells; cytology; drug effects; metabolism; Peptide Fragments; pharmacology; RNA, Messenger; genetics; metabolism; Rats; Signal Transduction; drug effects; rho-Associated Kinases; genetics
- From: Journal of Southern Medical University 2009;29(4):732-733
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of angiotensin II and angiotensin1-7 on alpha-smooth muscle actin (alpha-SMA)-induced Ca(2+)-independent pathways mediated by Rho kinase2 in hepatic stellate cells (HSCs).
METHODSHSC-T6 cells were treated with 10 micromol/L of AngII, Ang1-7, AngII +Ang1-7, and Ang1-7+A779. RT-PCR was used to detect the expression of Rho kinase2 (Rock2) in Ca(2+)-independent pathways, and alpha-SMA protein expression was detected by Western blotting.
RESULTSThe mRNA expression of Rock2 increased significantly in the cells after AngII treatment (P<0.01), but decreased following Ang1-7 treatment. Ang1-7 treatment significantly reduced alpha-SMA level in AngII-induced cells (P<0.01).
CONCLUSIONAng1-7 can inhibit AngII-induced activation of Rock2 and reduce alpha-SMA expression in HSCs.
