Recombinant adenovirus-mediated expression of 1.2-copy hepatitis B virus DNA in three hepatocytes.
- Author:
Wei-fang LIANG
1
;
Zhi-hua LIU
;
Kang-xian LUO
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Cell Line, Tumor; Culture Media, Conditioned; metabolism; DNA, Recombinant; genetics; DNA, Viral; genetics; metabolism; Gene Expression; Hepatitis B Antigens; metabolism; Hepatitis B virus; Hepatocytes; metabolism; virology; Humans; Reverse Transcriptase Polymerase Chain Reaction
- From: Journal of Southern Medical University 2009;29(4):738-740
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study hepatitis B virus (HBV) expression in 3 hepatocytes infected with recombinant adenovirus containing 1.2-copy HBV DNA.a
METHODSA chicken hepatoma cell line and two human hepatocytes were infected by the recombinant adenovirus containing 1.2-copy HBV DNA at 25 pfu/cell. HBV-specific mRNA was detected by RT-PCR 3 days after the infection, and HBsAg and HBeAg were detected by ELISA and HBV DNA by real-time PCR daily after the infection.
RESULTSHBV mRNA expression was detected in all the 3 cells after recombinant adenovirus infection, and the quantities of HBV DNA and HBV antigens in the culture supernatant increased with the passage of time.a
CONCLUSIONInfection with the recombinant adenovirus containing 1.2-copy HBV DNA can mediate HBV infection in the 3 cells in vitro.
