Detection of miR-122a and miR-224 expression in hepatocellular carcinoma by real-time fluorescence quantitative RT-PCR.
- Author:
Zhuo-ran LIU
1
;
Xiao-wei CHEN
;
Xin-hui QIAO
;
Rong WANG
;
Zheng XIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Base Sequence; Blotting, Northern; Carcinoma, Hepatocellular; diagnosis; genetics; Female; Gene Expression Regulation, Neoplastic; Humans; Liver Neoplasms; diagnosis; genetics; Male; MicroRNAs; genetics; Middle Aged; Reverse Transcriptase Polymerase Chain Reaction; Spectrometry, Fluorescence; Time Factors
- From: Journal of Southern Medical University 2009;29(4):751-753
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expressions of miR-122 and miR-224 in hepatocellular carcinoma (HCC) by real-time fluorescence quantitative RT-PCR and investigate the significance of miRNAs in early diagnosis of HCC.
METHOD2(-Delta Delta CT) method was used for quantitative analysis of the expression pattern of miR-122 and miR-224 in 35 HCC and adjacent normal tissues. All the quantitative results were confirmed by Northern blotting.
RESULTSCompared with adjacent normal tissues, the HCC tissues showed significant miR-122 down-regulation (P<0.01) and miR-224 over-expression (P<0.001).
CONCLUSIONSHCC has obvious alteration in the expression patterns of miR-122 and miR-224, and real-time fluorescence quantitative RT-PCR provide a new means for early, efficient, and accurate diagnosis of HCC.
