Small interference RNA targeting nuclear factor-kappaB inhibits endometriotic angiogenesis in chick embryo chorioallantocic membrane.
- Author:
Mu-biao LIU
1
;
Yuan-li HE
;
Jie ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chick Embryo; Chorioallantoic Membrane; blood supply; metabolism; Endometriosis; genetics; physiopathology; Female; Humans; NF-kappa B; deficiency; genetics; Neovascularization, Pathologic; genetics; RNA Interference; RNA, Small Interfering; genetics
- From: Journal of Southern Medical University 2009;29(4):757-759
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of small interference RNA (siRNA) targeting nuclear factor-kappaB (NF-kappaB) on endometriosis.
METHODThe eutopic endometrium of women with endometriosis were transplanted into the nonvascular region of 8-day-old chicken embryo chorioallantocic membrane (CAM), and the effects of NF-kappaB p65 siRNA on the vascularization and endometriotic lesion formation were tested with proper controls.
RESULTSTransplantation of the endometrium onto the CAM resulted in a strong angiogenic response in the chicken tissue. The angiogenesis was significantly reduced and endometriotic lesion formation significantly suppressed with siRNA targeting NF-kappaB in comparison with the control group.
CONCLUSIONSThe NF-kappaB pathway is involved in the development of endometriotic lesions in vitro, and NF-kappaB gene silencing reduces endometriotic angiogenesis and promotes cell apoptosis in the endometriotic lesions, suggesting that NF-kappaB might be a good target for endometriosis treatment.
