Inhibitory effects of RNA interference on MMP-24 expression and invasiveness of ovarian cancer SKOV(3) cells.
- Author:
Ya-ping LUO
1
;
Mei ZHONG
;
Li-ping WANG
;
Mei ZHONG
;
Gui-qin SUN
;
Jing LI
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Cell Line, Tumor; Female; Gene Expression Regulation, Neoplastic; Humans; Matrix Metalloproteinases, Membrane-Associated; deficiency; genetics; metabolism; Ovarian Neoplasms; genetics; pathology; Plasmids; genetics; RNA Interference; RNA, Messenger; genetics; metabolism; Transfection
- From: Journal of Southern Medical University 2009;29(4):781-784
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibitory effect of RNA interference (RNAi) on MMP-24 expression and invasiveness of ovarian cancer SKOV(3) cells.
METHODTwo pairs of small interfering RNA (siRNA) specific to MMP-24 mRNA were designed and transfected into SKOV(3) cells. RT-PCR and Western blotting were used to detect the mRNA and protein expressions of MMP-24, and the cell invasiveness was assessed using an in vitro invasion test.
RESULTSAfter transfection with siRNA, the mRNA and protein expression levels of MMP-24 were obviously reduced in SKOV(3) cells, which also showed significantly decreased invasiveness in vitro.
CONCLUSIONSMMP-24 gene silencing by RNAi can suppress the invasiveness of ovarian cancer SKOV(3) cells in vitro, which may provide a new therapeutic approach of ovarian cancer.
