Regulation of hepatic stellate cell activation by interleukin-10/platelet derived growth factor/mitogen-activated protein kinase pathway.
- Author:
Tao LI
1
;
Xi-Sheng LENG
;
Zhi-Zhong QIN
;
Sheng-Han SONG
;
Li ZHAO
;
Liang-Fa XIONG
;
Ji-Run PENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Cell Proliferation; drug effects; Hepatocytes; cytology; drug effects; Interleukin-10; pharmacology; Mitogen-Activated Protein Kinases; biosynthesis; Platelet-Derived Growth Factor; biosynthesis; Rats; Signal Transduction
- From: Chinese Journal of Hepatology 2005;13(1):35-37
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the regulatory effect of interleukin-10 (IL10) on the activation of hepatic stellate cells (HSC) through platelet derived growth factor (PDGF) and mitogen-activated protein kinase (MAPK) pathways.
METHODSHSC were divided randomly into 4 groups. Group 1 served as a control. HSC were incubated with 1 ng/ml, 5 ng/ml, and 25 ng/ml IL-10 in groups 2, 3 and 4. RT-PCR and western blot were used to detect the expression of PDGF and MAPK protein ERK and p38 and alpha-SMA.
RESULTSCompared with the control group, expressions of ERK, p38 and alpha-SMA of groups 2, 3 and 4 were significantly lower (F values were 240.47, 21.39, 28.86 respectively. IL-10 inhibited PDGF and MAPK protein ERK and p38 and alpha-SMA expression in a dose-dependent way.
CONCLUSIONIL-10 inhibits activation of HSC through the PDGF/MAPK pathway.
