Sustaining effect of gene-transferring hepatic stellate cell strain CFSC/HGF on hepatocytes development.
- Author:
Yun-Fang WANG
1
;
Xue NAN
;
Yan-Hua LI
;
Rui ZHANG
;
Wen YUE
;
Fang YAN
;
Xue-Tao PEI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Proliferation; Cells, Cultured; Coculture Techniques; Colony-Forming Units Assay; Gene Transfer Techniques; Hepatocyte Growth Factor; metabolism; Hepatocytes; cytology; Humans; Liver; cytology; Proto-Oncogene Proteins c-met; metabolism; Transfection
- From: Chinese Journal of Hepatology 2005;13(1):45-48
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the sustaining effects of gene-transferring hepatic stellate cell strain CFSC/HGF on the development of hepatocytes.
METHODSA CFSC/HGF strain, expressing HGF steadily and effectively was established by recombined retroviral vector pMSCV-HGF infection. Morphology and ultra structure of hepatocytes, albumin and urea production, as well as ICG uptake and excretion were studied continuously following the hepatocytes cultured on the CFSC/HGF feeder layers. Parallel group of collagen-dependent hepatocytes culturing and hepatocytes culturing on CFSC were also conducted. Semi-quantitative RT-PCR analysis was made to evaluate the expression of HGF receptor c-Met.
RESULTSThe hepatocytes cocultured on CFSC/HGF feeder layers had a higher survival rate, and the functions of albumin secretion and urea syntheses and ICG uptake and excretion, were superior to the other two culture methods. The result of RT-PCR indicated that the c-Met expressed on the CFSC/HGF coculturing hepatocytes was up-regulated 2.23 times.
CONCLUSIONGene-transferring hepatic stellate cell strain CFSC/HGF exhibited a remarkable sustaining effect on the hepatocytes development. The up-regulation of c-Met expressed on the surfaces of the hepatocytes induced by CFSC/HGF might play some part in this function.
