Distribution of hepatitis B virus genotypes in Hubei province and its clinical significance.
- Author:
Yan-Chang LEI
1
;
You-Hua HAO
;
Yong-Jun TIAN
;
Zhong-Ji FENG
;
Bao-Ju WANG
;
De-Ying TIAN
;
Xi-Ping ZHAO
;
Dong-Liang YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Carcinoma, Hepatocellular; virology; Carrier State; virology; China; Female; Genotype; Hepatitis B virus; genetics; Hepatitis B, Chronic; complications; virology; Humans; Liver Cirrhosis; virology; Liver Failure, Acute; virology; Liver Neoplasms; virology; Male; Middle Aged
- From: Chinese Journal of Hepatology 2005;13(2):109-112
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the distribution of hepatitis B virus genotype in Hubei province (China) and its clinical significance.
METHODSSerum samples from 190 HBV DNA positive patients with chronic HBV infection,including 52 asymptomatic HBV carriers (ASC), 56 chronic hepatitis (CH), 32 fulminant hepatic failure (FHF), 22 liver cirrhosis (LC), and 28 hepatocellular carcinoma (HCC) patients were collected and tested for HBV genotypes by type-specific primers.
RESULTSA simple and precise genotyping system based on PCR using type-specific primers was developed for the determination of genotypes of hepatitis B virus (HBV). Of the 190 patients, 140 (73.7%) were genotype B and 42 (22.1%) were genotype C. Genotype B was more prevalent in the FHF and HCC patients than in the ASC patients; the ALT value was significantly higher in genotype B than in genotype C patients. The rate of anti-HBe was significantly higher in genotype B than in genotype C except in the patients of the ASC group.
CONCLUSIONThe system we used seems to be a useful tool for the molecular diagnosis of HBV infection and for large-scale surveys. Genotype B, genotype C and BC combination exist in Hubei province, and genotype B is the major genotype in this area especially in FHF and HCC patients.
