Expressions of MIP-1alpha, MCP-1 and their receptors CCR-1, CCR-2 in chronic myeloid leukemia cells.
- Author:
Wei-Liang WANG
1
;
Ti SHEN
;
Yu-Rong HUI
;
Xi-Chun GU
;
Rong-Sheng LI
Author Information
1. Department of Hematology, Beijing Hospital, Ministry of Health, Beijing 100730, China. weiliangwang@hotmail.com
- Publication Type:Journal Article
- MeSH:
Chemokine CCL2;
biosynthesis;
genetics;
Chemokine CCL3;
Chemokine CCL4;
Humans;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
metabolism;
Macrophage Inflammatory Proteins;
biosynthesis;
genetics;
Receptors, CCR1;
Receptors, CCR2;
Receptors, Chemokine;
biosynthesis;
genetics;
Tumor Cells, Cultured
- From:
Journal of Experimental Hematology
2006;14(3):433-436
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore the expression of MIP-1alpha, MCP-1 and their receptors CCR-1, CCR-2 in bcr/abl fusion gene positive CML cells, and to study the effects of P210(bcr/abl) fusion protein tyrosine kinase on expression of MIP-1alpha, MCP-1 and their receptors CCR-1, CCR-2 mRNAs in chronic myeloid leukemia cells. The expression levels of MIP-1alpha, MCP-1 and their receptors CCR-1, CCR-2 mRNA were detected by semi-quantitative RT-PCR in bcr/abl negative cells, bcr/abl positive cells, and P210(bcr/abl)-Rb-C-Box positive cells. The results showed that MIP-1alpha and CCR-1 mRNAs were expressed in bcr/abl negative cells, but not in positive cells. Both MCP-1 and CCR-2 mRNA cannot be detected in both bcr/abl positive and negative cells. After inhibiting P210(bcr/abl) tyrosine kinase activity by Rb-C-Box, expressions of MIP-1alpha and CCR-1 mRNAs were restored to normal (similar to P210(bcr/abl) negative cells). It is concluded that P210(bcr/abl) fusion protein inhibits the expression of MIP-1alpha and CCR-1 in chronic myeloid leukemia cells, but does not inhibit MCP-1 and CCR-2 mRNA expressions in these leukemia cells.
