Detection of the labile iron pool in leukemia cells and its significance.
- Author:
Guo-Cun JIA
1
;
Ju GAO
;
Qing-Kui LIAO
;
Feng-Yi LI
;
Li-Xing YUAN
;
Bin HE
Author Information
1. Department of Pediatric Hematology, Zhengzhou Children Hospital, Zhengzhou 450053, China.
- Publication Type:Journal Article
- MeSH:
Cation Transport Proteins;
antagonists & inhibitors;
biosynthesis;
metabolism;
Deferoxamine;
pharmacology;
Fluoresceins;
Fluorescent Dyes;
HL-60 Cells;
Humans;
Iron;
metabolism;
Iron Chelating Agents;
analysis;
metabolism;
Iron-Regulatory Proteins;
metabolism;
K562 Cells
- From:
Journal of Experimental Hematology
2006;14(3):468-470
- CountryChina
- Language:Chinese
-
Abstract:
To explore a rapid and easy method to detect labile iron of pool (LIP) in cells, HL-60 and K562 cells were cultured at a concentration 1 x 10(6)/ml in RPMI 1640 containing 10% heat-inactivated fetal bovine serum. The iron deprivation was induced by adding desferrioxamine (DFO) 10 - 100 micromol/L for 0 - 48 hours. The intracellular LIP was measured by probe calcein-AM. Calcein fluorescence was monitored in 1420 multilabel counter. The results indicated that when HL-60 and K562 cells were incubated with different concentrations of DFO, the calcein fluorescence intensity was higher than that of control group at 12, 24 and 48 hours (P < 0.05). Fluorescence value of representing LIP in DFO groups was lower than that in the control group. In conclusion, DFO can decrease LIP in leukemia cells. The approach used in this study may provide a simple and reliable method for detection of intracellular iron homeostasis.
