Killing activity in DC and CIK co-culture against hepatocarcinoma cells.
- Author:
Bao-An CHEN
1
;
Man LI
;
Zai-Yang SUN
;
Cui-Ping LI
;
Chong GAO
;
Yun-Yu SUN
Author Information
1. Department of Hematology, Zhongda Hospital, Southeast University, Nanjing 210009, China. cba8888@hotmail.com
- Publication Type:Journal Article
- MeSH:
Carcinoma, Hepatocellular;
immunology;
pathology;
Cells, Cultured;
Coculture Techniques;
Cytotoxicity, Immunologic;
Dendritic Cells;
cytology;
immunology;
Humans;
Killer Cells, Lymphokine-Activated;
cytology;
immunology;
Liver Neoplasms;
immunology;
pathology
- From:
Journal of Experimental Hematology
2006;14(3):543-546
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the proliferation activities and phenotype changes of DC, CIK and DC-CIK, and their cytotoxicity against hepatocarcinoma cells in co-culture of DC with CIK. Peripheral blood mononuclear cells (PBMNC) were isolated from healthy adult donors. After incubation of PBMNC for 2 hours, DCs were induced from adherent cells by some cytokines and CIKs were generated from non-adherent cells. Mature DCs were harvested after incubation for 9 days, and then were co-cultured with CIK at ratio of 1:5 for 3 days. The cytotoxicity activity against SMMC-7721 hepatocellular carcinoma cell line was detected by MTT assay. The results showed that CIK cells were able to lyse SMMC-7721 hepatocellular carcinoma cells at low ratios of effector to target. This effect was significantly enhanced by co-culture with DCs. It is concluded that CIK cells have high lytic activity against 7721 hepatocellular carcinoma cell line, which can be enhanced by co-culture with DC. DC-CIK cells are highly effective immune cells.
