Anti-CD28 antibody costimulation enhances anti-CD3 antibody activating T cells and lowering TGF-beta expression in vitro.
- Author:
Qun LOU
1
;
Ming LÜ
;
Ming YU
;
Yan LI
Author Information
1. Department of Blood Transfusion, The General Hospital of PLA, Beijing 100853, China. luoqun@public.bta.net.cn
- Publication Type:Journal Article
- MeSH:
Antibodies, Monoclonal;
pharmacology;
CD28 Antigens;
immunology;
CD3 Complex;
immunology;
Down-Regulation;
Humans;
Leukocytes, Mononuclear;
immunology;
metabolism;
Lymphocyte Activation;
drug effects;
T-Lymphocytes;
immunology;
Transforming Growth Factor beta;
biosynthesis
- From:
Journal of Experimental Hematology
2006;14(3):547-551
- CountryChina
- Language:Chinese
-
Abstract:
In order to study how to activate T cells and their immunological characteristics, the anti-CD3 and anti-CD28 McAbs were used to stimulate PBMNC, then their related immunological changes, such as lymphocyte transformation function, the percentage of CD8(+)CD25(+) cells and TGF-beta expression were deleted by lymphocyte transformation assay, flow cytometry and RT-PCR respectively. The results showed that in costimulation with anti-CD28 antibody stimulation, the activity of anti-CD3 antibody was significantly enhanced, the ratio of CD8(+)CD25(+) cells of T cells was obviously increased, while TGF-beta expression was down-regulated. It was concluded that the anti-CD28 antibody costimulation could provide stimulatory signal II, which make T cells more active, while the expression of TGF-beta significantly down-regulated.
