Molecular basis of partial D phenotypes in Chinese.
- Author:
Jun-Jie WU
1
;
Xiao-Zhen HONG
;
Xian-Guo XU
;
Ji HE
;
Qi-Hua FU
;
Li-Xing YAN
Author Information
1. Institute of Transfusion Medicine, Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
Asian Continental Ancestry Group;
Base Sequence;
Humans;
Molecular Sequence Data;
Mutation;
Phenotype;
Rh-Hr Blood-Group System;
genetics;
immunology
- From:
Journal of Experimental Hematology
2006;14(3):587-591
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the molecular basis of partial D phenotypes in Chinese, D variants with weak D expression was screened by using indirect anti-human globulin test (IAT) method, the polymerase chain reaction-sequence specific primer (PCR-SSP) method was employed to amplify RHD specific exons and their flanking regions. The amplification products were sequenced directly to determine the molecular basis of D variants. The results showed that ten cases of partial D phenotypes, including one case of D Va (Kou.), one case of D Va (Hus.), one case of D Va-like (YH.), and seven cases of D VI type III, were detected from 22 cases of weak D phenotype respectively. All ten cases of partial D phenotypes had one RHD allele deleted. In conclusion, the molecular basis of ten cases of partial D phenotype was confirmed, including D Va (Kou.) and D Va-like (YH.) phenotypes reported firstly in Chinese population.
