Evaluation of two methods for counting residual white blood cells in thrombocytaphoresis concentrates.
- Author:
Yong-Jun WANG
1
;
Zhuo-Lan SHENG
;
Jian XU
;
Bo-Li HUANG
;
Qing-Feng HU
;
Wu-Wei FENG
Author Information
1. Blood Center of Zhejiang Province, Hangzhou 310006, China. wyjygm@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Blood Component Transfusion;
Evaluation Studies as Topic;
Flow Cytometry;
Humans;
Leukocyte Count;
methods;
Leukocyte Reduction Procedures;
methods;
Plateletpheresis
- From:
Journal of Experimental Hematology
2006;14(3):614-616
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate flow-cytometry and Nageotte method for counting residual WBC in thrombocytaphoresis concentrates, their accuracies were determined by dilution studies separately; the repeatability was determined by measuring the interassay coefficient of variation for 14 replicates of a sample with known leukocyte concentration. 102 samples of leukocyte-depleted thrombocytaphoresis concentrates were detected by the above mentioned two methods, and the results were compared each other. The results showed that no difference was observed between two methods over a range of leukocyte concentrations from 0.8 to 10 WBC/microl (P > 0.05). In conclusion, flow-cytometry and Nageotte methods can be used for quality control of WBC-reduced blood components.
