Technique of detection of hepatitis C core antigen used in safety blood transfusion.
- Author:
Ren-Nan YAO
1
;
Jian-Hui ZHANG
;
Xiao-Jing HUANG
;
Qin YANG
;
Qin CAO
;
Xu-Cheng JIANG
Author Information
1. Department of Transfusion, 97th Hospital of PLA, Xuzhou 221004, China. yaorn97@sohu.com
- Publication Type:Journal Article
- MeSH:
Blood Donors;
Blood Transfusion;
Enzyme-Linked Immunosorbent Assay;
methods;
Hepacivirus;
isolation & purification;
Hepatitis C Antigens;
blood;
Humans;
Reverse Transcriptase Polymerase Chain Reaction;
methods;
Viral Core Proteins;
blood
- From:
Journal of Experimental Hematology
2006;14(3):617-618
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the feasibility to screen donor with HCV infection by means of HCV-cAg ELISA. The first and repeat assays were performed for detection of serum anti-HCV in 8677 donor's serum specimens from January 2003 to December 2005. All serum anti-HCV specimens with positive anti-HCV from first and repeat assays were finally identified by using HCV-cAg ELISA and HCV RT-PCR methods. The results showed that only 5 serum specimens were positive anti-HCV by HCV-cAg ELISA identification in 29 specimens including 15 specimens with positive ant-HCV in first assays and 14 specimens with positive anti-HCV in repeat assays, the positive rate detected by HCV cAg ELISA was 17.24%. 5 serum specimens were positive anti-HCV by HCV RT-PCR detection also in 29 specimens mentioned above, the positive rate detected by HCV RT-PCR was 17.24% too. It is concluded that sensitivity of HCVcAg ELISA is similar to HCV RT-PCR and may be useful for the early diagnosis of hepatitic C or used as a reliable method to screen donor with HCV infection in blood transfusion medicine.
