Effects of D-limonene on leukemia cells HL-60 and K562 in vitro.
- Author:
Xiao-Ming GUO
1
;
Qing LU
;
Zheng-Juan LIU
;
Li-Feng WANG
;
Bing-An FENG
Author Information
1. Department of Pediatrics, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Proliferation;
drug effects;
Cyclohexenes;
pharmacology;
HL-60 Cells;
Humans;
K562 Cells;
Mutation;
Proto-Oncogene Proteins c-bcl-2;
genetics;
Terpenes;
pharmacology;
Tumor Suppressor Protein p53;
genetics;
bcl-2-Associated X Protein;
genetics
- From:
Journal of Experimental Hematology
2006;14(4):692-695
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of D-limonene (D-L) on the cell growth and apoptosis in HL-60, K562 cells and to elucidate its mechanism, the influence of D-L on proliferation of HL-60 and K562 cells was determined by propidium iodide assay, the expression levels of mutant p53, bcl-2, bax gene were detected by cell morphological analysis, flow cytometry and immunohistochemistry staining, the D-L-inducing HL-60 and K562 cell apoptosis in vitro was observed systematically. The results showed that D-L inhibited HL-60 and K562 cell growth in a dose- and time-dependent manner with the IC50 of 0.75 mmol/L similarly, D-L induced apoptosis of HL-60 and K562 cells, and expression of bcl-2 gene was down regulated by D-L in a concentration-dependent manner in HL-60 cells. The bcl-2, mutant type of p53 genes were down regulated while bax gene was up regulated by D-L in a concentration-dependent manner in K562 cells. It is concluded that D-L can inhibit proliferation and induce apoptosis of HL-60 and K562 cells. The bcl-2, mutant type of p53 and bax may be involved in the gene regulation of D-L-induced apoptosis.
