Growth, immunophenotype and interleukin-6 level of bone marrow stromal cells in patients with multiple myeloma and acute leukemia.
- Author:
Jun-Ling ZHUANG
1
;
Xuan WANG
;
Jie-Ping ZHANG
;
Yong-Ji WU
Author Information
1. Department of Hematology, Peking Union Hospital, Beijing 100730, China. em-myzhuang@sina.com
- Publication Type:Journal Article
- MeSH:
Acute Disease;
Bone Marrow Cells;
immunology;
metabolism;
pathology;
Cell Proliferation;
Humans;
Hyaluronan Receptors;
analysis;
Immunophenotyping;
Integrin beta1;
analysis;
Interleukin-6;
analysis;
Leukemia;
immunology;
metabolism;
pathology;
Multiple Myeloma;
immunology;
metabolism;
pathology;
Stromal Cells;
immunology;
metabolism;
pathology;
Tumor Cells, Cultured
- From:
Journal of Experimental Hematology
2006;14(4):700-703
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the growth, immunophenotype and interleukin-6 (IL-6) level of bone marrow stromal cells (BMSC) in patients with acute leukemia (AL) and multiple myeloma (MM). BMSC was cultured by wall-adhesion method and the growth of BMSC was observed. The immunophenotype and cell cycle of BMSC were detected by flow cytometry. The level of interleukin 6 (IL-6) in BMSC culture system was detected by ELISA. The results showed that the primary (17.3 +/- 7.8 days) and continuous (10.3 +/- 3.5 days) growth cycle of BMSC in patients with AL were significantly shorter than those in patients with MM (26.5 +/- 6.3 and 16.5 +/- 4.1 days respectively), and shorter than those in normal controls (25.8 +/- 6.3 and 17.5 +/- 2.4 days) respectively. Similarly, S + G2% (17.4 +/- 3.6%) of BMSC in patients with AL was significantly higher than those in patients with MM (8.5 +/- 2.2%) and in normal controls (8.9 +/- 2.3%). All of the three groups showed positive antigen expressions with CD29 and CD44 were 100%, while CD138, CD34, CD54, CD56 positive were not expressed and CD106 was partially expressed positive. The supernatant IL-6 level of BMSC system in MM patients (1288.5 +/- 736.7 pg/ml) was significantly higher than those in AL patients (859.3 +/- 203.1 pg/ml) and normal controls (850.9 +/- 129.5 pg/ml). It is concluded that the growth, S + G2% of cell cycle and IL-6 level of BMSC in patients with MM, AL and normal control are significantly different, whereas the antigen expressions are similar.
